Molecular biotechnology

Recombinant DNA technology, Commercialization of molecular biotechnology products, Concerns

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Recombinant DNA technology

Restriction endonucleases, Ligation

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Plasmid cloning vectors

Plasmid cloning vectors, Plasmid cloning vector pBR322, Other plasmid cloning vectors

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Plasmid cloning vectors

Cosmids, Types of cloning vectors

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Creating and screening a library

Making a genomic library, Screening by DNA hybridization / immunological assay / protein activity

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Creating and screening a library

Cloning DNA sequences that encode eukaryotic proteins

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Chemical synthesis of DNA

Chemical synthesis of DNA

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Chemical synthesis of DNA

Uses of synthesized oligonucleatides

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Polymerase Chain Reaction

Polymerase Chain Reaction

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DNA sequencing techniques

DNA sequencing techniques

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Sequencing and imaging

Cyclic reversible termination

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Sequencing and imaging

Sequencing by ligation, Pyrosequencing

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Sequencing and imaging, Functional genomics

Real-time sequencing, GenBank sequence depository

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Functional genomics

Functional genomics, DNA microarray

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Functional genomics

Senal analysis of gene expression(SAGE)

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Proteomics

Protein expression profiling: 2D differential in-gel electrophoresis & ICAT, Protein microarrays

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Manipulation of gene expression

Basic features of gene expression control, Promoter control

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Manipulation of gene expressionFusion Proteins

regulatable promoters, pCP3 expression system, Sigma factor

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Fusion proteins

Use of fusion protein, Cleavage of fusion protein

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other factors attecting gene expression

Increasing protein stability, Increasing secretion, Metabolic load

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Directed mutagenesis

Oligonucleotide-directed mutagenesis with plasmid, PCR amplified oligonucieotide directed mutagenesis

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Examples of protein engineering

protein engineering introduction, Adding disulfide bonds, Increasing enzymatic activity, Decreasing protease sensitivity, Increasing enzyme stability and specificity

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