분자생물학 개론 및 실습

강의상세

생명과학의 가장 중심이 되는 분자생물학 전반에 관한 개론적인 내용을 학습한다. 주로 핵산과 관련하여 DNA 복제의 기전, 세포의 유전자 발현 조절, 유전자 재조합 기술 등을 다룬다.

1.	Introduction	Introduction to Molecular Biology -Syllabus - Historical Background.
2.	Macromolecules	Proteins, Nucleic acid, polysaccharide, and lipid. Structure and Function of macromolecules.
3.	Structure of Macromolecules: nucleic acid	Structure and Function of Proteins. Structure and Function of Nucleic acids - On the biological membrane.
4.	Genetic Material	Experimental evidances that DNA is the Genetic material. Characteristics of Genetic material.
5.	DNA Replication	Basic mechanism of DNA. Enzymologies for DNA replication.
6.	Mutageneis	Types of mutagenesis. Spontaneous mutagenesis and induced mutagenesis. Ames test.
7.	DNA Repair	types of DNA repair. Direct repair. Excision repair, SOS repair.
8.	Transcription	RNA polymerase. Elements for transcription. Stages of Transcription.
9.	Gene Expression(1)	concepts for gene expression. diauxic growth curve.one the lac operon.
10.	Gene Expression(2)	operon.arabinose operon.trp operon.
11.	Translation(1); Protein synthesis	Elements for translation.Genetic code. Characteristics of genetic code.
12.	Translation(2); Protein synthesis	Elements for translation. mRNA. ribosome . tRNA .amino acids.
13.	Translation(3); Protein synthesis	Stages of translation. initiation; SD sequence, P site, A-site, fMet-tRNA, IFs - elongation; transpeptidation, translocation, G-protein, EFs - termination; stop codons, RFs, RRF
14.	Genomics and Applications of gene manipulation	Introduction to Genomics - Overview of gene manipulation.Applications of genetic engineering.