| 1. |
 |
Introduction to the course (Prof. Marina Hincapie) |
Syllabus: reading lists, references in library, exams, presentations and grades. General overview of protein chemistry and post translational modifications. |
 |
| 2. |
|
The production of recombinant DNA-derived proteins in bacterial and mammalian cell systems. The isolation, purification and testing procedures required for the production of a pure glycoprotein standard or pharmaceutical. Regulatory issues and discussion of the balance between safety and efficacy/purity (risk to benefit analysis). (Prof. Marina Hincapie) |
Methods for enrichment and isolation of target glycoproteins |
|
| 3. |
|
Heterogeneity of protein pharmaceuticals, product variants, host cell contaminantsSummary of chromatographic approaches to the study of protein structures: HPLC and capillary electrophoresis, SDS-PAGE, 1 and 2D gel electrophoresis, IEF (conventional and immobilized pH gradients), mass spectrometry detection, affinity chromatography, peptide mapping, separation of protein isoforms. |
Introduction of chromatography methods for protein separation and isoaltion |
|
| 4. |
|
Aggregation, mechanism of formation, detection and quantitation – size exclusion chromatography, SDS-PAGE, fluorescence spectroscopy, hydrophobic interaction chromatography, laser light scattering, freezing and shaking studies, formulation in detergent, examples trypsin inhibitor, platelet – derived growth factor, fibroblast growth factor. Overview of protein degradation reactions, backbone cleavage, crosslinking, glycation and other carbonyl adducts, immunogenicity, formulation, product stability. |
Introduction of mechanisma for protein aggregation |
|
| 5. |
|
Analytical methods for the measurement of the heterogeneity of proteins, product variants and biological sample effects. |
Introduction of Analytical methods for the measurement of the heterogeneity of proteins, product variants and biological sample effects. |
|
| 6. |
|
Glycoproteins, biosynthetic pathways, protein heterogeneity, biological and medical effects, separations of isoforms and structure determination, HPLC and capillary separations. |
Introduction of glycoproteomics |
|
| 7. |
|
Phosphoproteins, biosynthetic pathways, protein heterogeneity, biological and medical effects, separations of isoforms and structure determination, HPLC and capillary separations |
Introduction of phosphoproteomics |
|
| 8. |
|
Case study: The characterization of isoforms of human growth hormone. |
Introduction of Analytical methods for the characterization of isoforms fo human growth hormnone |
|
| 9. |
|
Proteomics and the study of human disease and chromosomes |
Introduction of analytical method for proteomic study of human disease and chromosomes |
|
대구광역시 동구 동내로 64(동내동 1119) 우)41061
페이스북
트위터
카카오톡
네이버밴드
링크복사
