생분석화학

울산과학기술원
Steven A. Soper

페이스북 트위터 카카오톡 네이버밴드 링크복사

주제분류

공학 >정밀ㆍ에너지 >나노공학
등록일자

2010.08.13

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3,292

Provide to the student information on the basic bioanalytical strategies used to perform molecular analysis. The analysis will be focused on biopolymers such as nucleic acids (DNA and RNA) and proteins. There will be 3 general sections to this class: (1) Introduction to biopolymer structure; (2) Macro-scale techniques for elucidating molecular structure; (3) Micro- and nano-scale techniques for analyzing molecular structure. Specific topical areas that will be covered include PCR technologies, micro-scale bioseparations (multi-dimensional as well), DNA/protein microarrays, DNA sequencing and genotyping and immunoassays. Examples using the technologies introduced to the student will come from such areas as DNA forensics, molecular diagnostics and proteomics.

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Section 1Introduction to biopolymer structure and functiona. Nucleic acid structure and function b. Protein structure and functionc. ing nucleic acids to proteins: Functional biology, flow of genetic information, tranion and translation

차시별 강의

1.		Section 1 Introduction to biopolymer structure and function a. Nucleic acid structure and function b. Protein structure and function c. Linking nucleic acids to proteins: Functional biology, flow of genetic information, transcription and translation Introduction to biopolymer structure and function a. Nucleic acid structure and function b. Protein structure and function c. Linking nucleic acids to proteins: Functional biology, flow of genetic information, transcription and translation
2.		Section 1 Introduction to biopolymer structure and function a. Nucleic acid structure and function b. Protein structure and function c. Linking nucleic acids to proteins: Functional biology, flow of genetic information, transcription and translation
3.		Section 1 Introduction to biopolymer structure and function a. Nucleic acid structure and function b. Protein structure and function c. Linking nucleic acids to proteins: Functional biology, flow of genetic information, transcription and translation
4.		Section 1 Introduction to biopolymer structure and function a. Nucleic acid structure and function b. Protein structure and function c. Linking nucleic acids to proteins: Functional biology, flow of genetic information, transcription and translation
5.		Section 1Introduction to biopolymer structure and functiona. Nucleic acid structure and function b. Protein structure and functionc. ing nucleic acids to proteins: Functional biology, flow of genetic information, tranion and translation
6.		Section IIMacro-scale techniques for elucidating molecular structure and function;a. electrophoresis for DNA analysis; slab gel electrophoresis, ds vs ss DNA separationsb. Polymerase Chain Reaction (PCR), and isothermal amplification techniques (RCT, SDA, etc)c. DNA sequencing (Sanger and SbS)d. DNA/Protein Microarrayse. Restriction mapping and cloningf. Mutational Analysis of DNAg. Proteomics (Top-down, Bottom-up and Shotgun)h. Immunoassays
7.		Section II Macro-scale techniques for elucidating molecular structure and function; a. electrophoresis for DNA analysis; slab gel electrophoresis, ds vs ss DNA separations b. Polymerase Chain Reaction (PCR), and isothermal amplification techniques (RCT, SDA, etc) c. DNA sequencing (Sanger and SbS) d. DNA/Protein Microarrays e. Restriction mapping and cloning f. Mutational Analysis of DNA g. Proteomics (Top-down, Bottom-up and Shotgun) h. Immunoassays
8.		Section IIMacro-scale techniques for elucidating molecular structure and function;a. electrophoresis for DNA analysis; slab gel electrophoresis, ds vs ss DNA separationsb. Polymerase Chain Reaction (PCR), and isothermal amplification techniques (RCT, SDA, etc)c. DNA sequencing (Sanger and SbS)d. DNA/Protein Microarrayse. Restriction mapping and cloningf. Mutational Analysis of DNAg. Proteomics (Top-down, Bottom-up and Shotgun)h. Immunoassays
9.		Section IIMacro-scale techniques for elucidating molecular structure and function;a. electrophoresis for DNA analysis; slab gel electrophoresis, ds vs ss DNA separationsb. Polymerase Chain Reaction (PCR), and isothermal amplification techniques (RCT, SDA, etc)c. DNA sequencing (Sanger and SbS)d. DNA/Protein Microarrayse. Restriction mapping and cloningf. Mutational Analysis of DNAg. Proteomics (Top-down, Bottom-up and Shotgun)h. Immunoassays
10.		Section IIMacro-scale techniques for elucidating molecular structure and function;a. electrophoresis for DNA analysis; slab gel electrophoresis, ds vs ss DNA separationsb. Polymerase Chain Reaction (PCR), and isothermal amplification techniques (RCT, SDA, etc)c. DNA sequencing (Sanger and SbS)d. DNA/Protein Microarrayse. Restriction mapping and cloningf. Mutational Analysis of DNAg. Proteomics (Top-down, Bottom-up and Shotgun)h. Immunoassays
11.		Section IIMacro-scale techniques for elucidating molecular structure and function;a. electrophoresis for DNA analysis; slab gel electrophoresis, ds vs ss DNA separationsb. Polymerase Chain Reaction (PCR), and isothermal amplification techniques (RCT, SDA, etc)c. DNA sequencing (Sanger and SbS)d. DNA/Protein Microarrayse. Restriction mapping and cloningf. Mutational Analysis of DNAg. Proteomics (Top-down, Bottom-up and Shotgun)h. Immunoassays
12.		Section IIIMicro- scale techniques for analyzing molecular structurea. Micro scale PCRb. Micro scale capillary electrophoresis- DNA separation- DNA sequencingc. Solid phase bioreactors (solid support, attachment chemistries, extraction DNA, proteolytic digestion of proteins)d. Microchip arrays (DNA and Protein)e. Integrated micro-systems for Performing Molecular Analysisf. Nanotechnology – how can it be used for molecular analysis
13.		Section IIIMicro- scale techniques for analyzing molecular structurea. Micro scale PCRb. Micro scale capillary electrophoresis- DNA separation- DNA sequencingc. Solid phase bioreactors (solid support, attachment chemistries, extraction DNA, proteolytic digestion of proteins)d. Microchip arrays (DNA and Protein)e. Integrated micro-systems for Performing Molecular Analysisf. Nanotechnology – how can it be used for molecular analysis
14.		Section IIIMicro- scale techniques for analyzing molecular structurea. Micro scale PCRb. Micro scale capillary electrophoresis- DNA separation- DNA sequencingc. Solid phase bioreactors (solid support, attachment chemistries, extraction DNA, proteolytic digestion of proteins)d. Microchip arrays (DNA and Protein)e. Integrated micro-systems for Performing Molecular Analysisf. Nanotechnology – how can it be used for molecular analysis
15.		Section IIIMicro- scale techniques for analyzing molecular structurea. Micro scale PCRb. Micro scale capillary electrophoresis- DNA separation- DNA sequencingc. Solid phase bioreactors (solid support, attachment chemistries, extraction DNA, proteolytic digestion of proteins)d. Microchip arrays (DNA and Protein)e. Integrated micro-systems for Performing Molecular Analysisf. Nanotechnology – how can it be used for molecular analysis