유전공학

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자연과학 >생물ㆍ화학ㆍ환경 >생명공학
강의학기

2017년 1학기

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강의계획서

유전공학은 현대 생명공학에 가장 많이 이용되어지고 있는 생명공학기법중에 하나로써 유전자 시퀀싱과 재조합 기술을 이용하여 DNA를 조작하여 생물체에 새로운 형질을 부여하거나 기존의 형질을 향상시키는 응용학문이다. 본 과목에서는 이러한 유전공학적 기술에 필요한 기본적인 지식을 습득하고 이를 바탕으로 기존의 예를 통하여 유전공학기술을 이용하여 새로운 의약품 또는 의약품의 대량생산에 활용할 수 있는 능력을 배양하는 것을 목표로 한다.

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Molecular biotechnology

차시별 강의

1.	Molecular biotechnology	Recombinant DNA technology, Commercialization of molecular biotechnology products, Concerns
2.	Recombinant DNA technology	Restriction endonucleases, Ligation
	Plasmid cloning vectors	Plasmid cloning vectors, Plasmid cloning vector pBR322, Other plasmid cloning vectors
3.	Plasmid cloning vectors	Cosmids, Types of cloning vectors
	Creating and screening a library	Making a genomic library, Screening by DNA hybridization / immunological assay / protein activity
4.	Creating and screening a library	Cloning DNA sequences that encode eukaryotic proteins
	Chemical synthesis of DNA	Chemical synthesis of DNA
5.	Chemical synthesis of DNA	Uses of synthesized oligonucleatides
	Polymerase Chain Reaction	Polymerase Chain Reaction
6.	DNA sequencing techniques	DNA sequencing techniques
7.	Sequencing and imaging	Cyclic reversible termination
	Sequencing and imaging	Sequencing by ligation, Pyrosequencing
8.	Sequencing and imaging, Functional genomics	Real-time sequencing, GenBank sequence depository
	Functional genomics	Functional genomics, DNA microarray
9.	Functional genomics	Senal analysis of gene expression(SAGE)
	Proteomics	Protein expression profiling: 2D differential in-gel electrophoresis & ICAT, Protein microarrays
10.	Manipulation of gene expression	Basic features of gene expression control, Promoter control
11.	Manipulation of gene expressionFusion Proteins	regulatable promoters, pCP3 expression system, Sigma factor
	Fusion proteins	Use of fusion protein, Cleavage of fusion protein
12.	other factors attecting gene expression	Increasing protein stability, Increasing secretion, Metabolic load
13.	Directed mutagenesis	Oligonucleotide-directed mutagenesis with plasmid, PCR amplified oligonucieotide directed mutagenesis
14.	Examples of protein engineering	protein engineering introduction, Adding disulfide bonds, Increasing enzymatic activity, Decreasing protease sensitivity, Increasing enzyme stability and specificity